Ubiquitin-Vinyl Sulfone

Catalog number: SBB-PS0032, 50 μg

Ubiquitin-Vinyl Sulfone is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Sulfone group. Ub-Vinyl Sulfone can be used for activity profiling experiments and DUB inhibitor specificity determination. It targets four of the five major DUB families: UCH, USP, OUT, and MJD, while leaving JAMM metalloproteases uninhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Vinyl Sulfone concentration of 1-5 μM.

In stock
SKU
0032
$169.00

Description

Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways; it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a c-terminal warhead (Vinyl Sulfone). Ubiquitin-Vinyl Sulfone is a potent, irreversible, and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Sulfone group. Ub-Vinyl Sulfone can be used for activity profiling experiments and determining DUB inhibitor specificity. It targets three of the four major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Vinyl Sulfone concentration of 1-5 μM.

For Research Use Only, Not For Use In Humans.

Specifications

Product Overview
Quantity: 50 μg
Molecular Weight: 8.6 kDa
Purity: >97% by LCMS
Label or Dye: Vinyl Sulfone
Substrate Properties: Protein-Based Substrate. Typical experimental concentration 1-5 μM.
Storage Buffer: 50mM MES pH 6.0, 100mM NaCl
Storage Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.

Figures & Data

UCHL3 + Ub-AMC + Ubiquitin-Vinyl Sulfone

Figure 1. Kinetic Activity. UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 12.5 nM and 0.1 nM Ubiquitin Vinyl Sulfone.

Citations & References

1) Borodovsky et al. 2001, 20, 5187.

2) Xu et al. RSC Adv 2016, 6, 
47926. 


3) Tong, X. et al. (2012) J. Biol. Chem. 287:25280. Galardy et al. Methods in Enzymology 2005, 399, 120.