Ubiquitin-Vinyl Methyl Ester
Catalog number: SBB-PS0033, 50 μg
Ubiquitin-Vinyl Methyl Ester is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Methyl Ester group. Ub-Vinyl Methyl Ester can be used for activity profiling experiments and DUB inhibitor specificity determination. It targets four of the five major DUB families: UCH, USP, OUT, and MJD, while leaving JAMM metalloproteases uninhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin- Vinyl Methyl Ester concentration of 1-5 μM.
Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways; it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a c-terminal warhead (Vinyl Methyl Ester). Ubiquitin-Vinyl Methyl Ester is a potent, irreversible, and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Methyl Ester group. Ub-Vinyl Methyl Ester can be used for activity profiling experiments and determining DUB inhibitor specificity. It targets three of the four major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Vinyl Methyl Ester concentration of 1-5 μM.
|Molecular Weight:||8.6 kDa|
|Purity:||>97% by LCMS|
|Label or Dye:||Vinyl Methyl Ester|
|Substrate Properties:||Protein-Based Substrate. Typical experimental concentration 1-5 μM.|
|Storage Buffer:||50mM MES pH 6.0, 100mM NaCl|
|Storage||Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.|
Figures & Data
UCHL3 + Ub-AMC + Ubiquitin-Vinyl Methyl Ester
Figure 1. Kinetic Activity. UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 15.0 nM and 0.1 nM Ubiquitin Vinyl Methyl Ester.
Certificates of Analysis (COA)
Citations & References
2) Xu et al. RSC Adv 2016, 6, 47926.
3) Tong, X. et al. (2012) J. Biol. Chem. 287:25280. Galardy et al. Methods in Enzymology 2005, 399, 120.