Cryptate Donors
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Europium-Cryptate Ubiquitin, human recombinant$2,985.00Catalog number: SBB-TR0014, 20 μg
Highly purified recombinant protein site-specifically labeled with a single Europium Cryptate moiety. All lysines are available along with a fully functional C-terminus, making the Europium Cryptate Ub ideal for chain formation using the the E1 – E2 and E3 conjugation cascade. The Cryptate has an excitation maximum of 304nm and an emission maximum of 620nm. This protein was expressed in E.coli.[1][2][3][4]
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100x TRF-Ubiquitin Mix$3,475.00Catalog number: SBB-TR0051-2K, 2000 wells
Mixture of Europium Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) combined in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Conjugation reactions are monitored at Excitation = 304 nm, Emission channel 1 = 620 nm, Emission channel 2 = 665 nm. These proteins were expressed in E.coli.
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