TR-FRET

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  1. Europium-Cryptate Ubiquitin, human recombinant
    $495.00

    Catalog number: SBB-TR0014, 20 μg

    Highly purified recombinant protein site-specifically labeled with a single Europium Cryptate moiety. All lysines are available along with a fully functional C-terminus, making the Europium Cryptate Ub ideal for chain formation using the the E1 – E2 and E3 conjugation cascade. The Cryptate has an excitation maximum of 304nm and an emission maximum of 620nm. This protein was expressed in E.coli.[1][2][3][4]

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  2. 100x TRF-Ubiquitin Mix
    $495.00

    Catalog number: SBB-TR0051-2K, 2000 wells

    Mixture of Europium Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) combined in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Conjugation reactions are monitored at Excitation = 304 nm, Emission channel 1 = 620 nm, Emission channel 2 = 665 nm. These proteins were expressed in E.coli.

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  3. MDM2 E3 Ligase TR-FRET Kit
    $595.00

    Catalog number: SBB-KF0030, 400 wells

    The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto MDM2 leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).These proteins were expressed in E.coli.

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  4. ITCH E3 Ligase TR-FRET Kit
    $595.00

    Catalog number: SBB-KF0035, 400 wells

    The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto ITCH leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

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  5. Parkin E3 Ligase TR-FRET Kit
    $595.00

    Catalog number: SBB-KF0036, 400 wells

    The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto Parkin leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

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  6. NEDD4 E3 Ligase TR-FRET Kit
    $595.00

    Catalog number: SBB-KF0056, 400 wells

    The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto NEDD4 leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

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  7. XIAP E3 Ligase TR-FRET Kit
    $595.00

    Catalog number: SBB-KF0057, 400 wells

    The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto XIAP leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

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  8. Cullin4a/Rbx1/DDB1/CRBN – E3 Ligase Complex TR-FRET Kit
    $595.00

    Catalog number: SBB-KF0120, 400 wells

    The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto CRBN leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).

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  9. Cy5-Ubiquitin, human recombinant
    $139.00

    Catalog number: SBB-TR0015, 50 μg

    Highly purified recombinant protein, which has been labeled with a single Cyanine5 (Cy5) moiety at a specific site that keeps all lysines within the protein available and has a fully functional C-terminus that allows for further conjugation into ubiquitin chains and does not affect or impairs the E1 – E2 and E3 conjugation cascade. Cy5-Ubiquitin has an excitation maximum Ex646nm and an emission maximum Em662nm. This protein was expressed in E.coli.[1][2][3][4]

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  10. Fluorescein-Ubiquitin (6-FAM-Ub), human recombinant
    $139.00

    Catalog number: SBB-TR0016, 50 μg

    Highly purified recombinant protein, which has been labeled with a single fluorescein (6-FAM) moiety at a specific site that keeps all lysines within the protein available and has a functional C-terminus.  Allows for conjugation into ubiquitin chains and does not affect or impair the E1 – E2 and E3 conjugation cascade. 6-FAM Ubiquitin has an excitation maximum Ex494nm and an emission maximum Em520nm.This protein was expressed in E.coli.[1][2][3]

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