Suc-Leu-Leu-Val-Tyr-AMC (LLVY-AMC)
Catalog number: SBB-PS0010, 2 mg
A Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome, calpains and other chymotrypsin-like proteases (Ex=345 nm and Em=445 nm).[1][2][3][4]
In stock
SKU
0010
$65.00
Description
Suc-LLVY-AMC a 7-amino-4-methylcoumarin (AMC) labeled fluorogenic substrate can be used to measure the chymotrypsin–like activity of the 20S and 26S proteasome as well as calpains and other chymotrypsin-like proteases. Working concentrations of this substrate is 20-50 µM. Cleavage of the peptide by the enzymes liberates the fluorophore AMC which generates strong increase in fluorescence fluorescent that can be monitored fluorimetrically at Ex=345 nm and Em=445 nm.
For Research Use Only, Not For Use In Humans.
Specifications
| Quantity: | 2.0 mg |
|---|---|
| Molecular Weight: | 763.9 Da |
| Purity: | >99% by HPLC |
| Readout: | Endpoint / Kinetic |
| Label or Dye: | 7-Amino-4-methylcoumarin |
| Detection Method: | Fluorescence |
| Substrate Properties: | Protein-Based Substrate, C40H53N5O10 , CAS#94367-21-2 |
| Excitation/Emission (nm): | 345/445 |
| Storage Buffer: | Lyophilized |
| Storage | Store at 4°C after product arrival. After preparing a stock in DMSO ( ≥10 mM) store product at -20°C to −80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. |
Figures & Data
Suc-LLVY-AMC
Figure 1. Suc-LLVY-AMC, Chemical Structure.
Structure of Suc_LLVY-AMC, CAS # 94367-21-2
Immunoproteasome Activity
Figure 2. 20S Immunoproteasome vs. 20S Constitutive Proteasome Activity.
LLVY-AMC exhibits a high specific activity for both immuno and constitutive proteasome compared to other substrates. Specific activities are higher when using immunoproteasome.
Certificates of Analysis (COA)
Citations & References
1) Dammer, E. et al. (2011). Polyubiquitin Linkage Profiles in Three Models of Proteolytic Stress Suggest the Etiology of Alzheimer Disease. J Biol Chem doi: 10.1074/jbc.M110.149633.
2) Gallery, M. et al. (2007). The JAMM motif of human deubiquitinase Poh1 is essential for cell viability. Mol Cancer Ther 6, 262.
3) Cardiac proteasome functional insufficiency plays a pathogenic role in diabetic cardiomyopathy: J. Li, et al.; J. Mol. Cell. Cardiol. 102, 53 (2016), Abstract;
