His6-SENP1, human recombinant

Catalog number: SBB-DE0026, 50 μg

Purified recombinant human Sentrin Specific Protease 1 (SENP1, catalytic domain) is a SUMO-specific enzyme that catalyzes two essential functions. Processing the full-length versions of SUMO1, SUMO2, and SUMO3 into its mature forms and the deconjugation of SUMOylated proteins. This SENP1 is N-terminally His6 tagged and expressed in E.coli.[1][2][3][4]

In stock
SKU
0026
$149.00

Description

SUMO/Sentrin Specific Peptidase 1 (SENP1) is a member of the SENP family of proteases, which belong to the group of cysteine­type peptidases that either catalyze the mature form of full­-length Small Ubiquitin­related MOdifiers (SUMOs), or the deconjugation of SUMOs from SUMOylated proteins, specifically SUMO2/3 chains by removing the distal SUMO1. SENP1 is expressed in many organs and is localized in the nucleus as well as the cytoplasm. SENP1 is thought to play a role in the development, progression, and metastasis of prostate cancer and has been shown to regulate erythropoietin production during hypoxia by regulating the stability of HIF1α. Furthermore, SENP1 deconjugates SUMO1 from HIPK2, from HDAC1, and BHLHE40/DEC1, which decreases its transcriptional repression activity. SENP1 has been shown to desumoylate CCAR2, which subsequently decreases the interaction with SIRT1.

For Research Use Only, Not For Use In Humans.

Specifications

Product Overview
Quantity: 50 μg
Molecular Weight: 31.5 kDa
Purity: > 95% by SDS-PAGE
Substrate Properties: His6-tagged, N-terminus. Working concentrations of this enzyme range from 1 to 10 nM.
Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol, 1 mM TCEP
Storage Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.

Figures & Data

His6-SENP1, human recombinant

Figure 1. His6-SENP1, SDS-PAGE From left to right, increasing amounts of His6-SENP1 loaded onto a 4-12% SDS-PAGE gel, stained with coomassie blue.

Citations & References

1) Bailey, D. and P. O'Hare. "Characterization Of The Localization And Proteolytic Activity Of The SUMO-Specific Protease, SENP1". Journal of Biological Chemistry 279.1 (2003): 692-703. Web.

2) Kim, Young Ho et al. "Desumoylation Of Homeodomain-Interacting Protein Kinase 2 (HIPK2) Through The Cytoplasmic-Nuclear Shuttling Of The SUMO-Specific Protease SENP1". FEBS Letters 579.27 (2005): 6272-6278. Web. 8 Mar. 2017.

3) Scherer, Steven E. et al. "The Finished DNA Sequence Of Human Chromosome 12". Nature 440.7082 (2006): 346-351. Web. 8 Mar. 2017.

4) Sung, Ki Sa et al. "Differential Interactions Of The Homeodomain-Interacting Protein Kinase 2 (HIPK2) By Phosphorylation-Dependent Sumoylation". FEBS Letters 579.14 (2005): 3001-3008. Web. 8 Mar. 2017.