His6-Poly-Ubiquitinated p53, human recombinant

Catalog number: SBB-US0012, 20 μg

This product consists of His6-p53 protein expressed in E.coli and ubiquitinated by MDM2, and subsequently purified from conjugation-reaction proteins/ enriched post-conjugation. Western blot analysis shows multiple states of poly-ubiquitination, and digestion with USP7 shows ubiquitinated p53 can be deconjugated. [1][2]

In stock
SKU
0012
$289.00

Description

p53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. E3 ligase MDM2 mediated ubiquitin conjugation of p53 and subsequent p53 down-regulation via proteasomal degradation interrupts this crucial role, contributing to tumorogenesis (and cancer). This product consists of His6-p53 protein ubiquitinated by MDM2, and subsequently purified from conjugation-reaction proteins/ enriched post-conjugation. Western blot analysis shows multiple states of poly-ubiquitination, and deconjugation with USP7 shows purified ubiquitinated p53 can function as a native substrate.

For Research Use Only, Not For Use In Humans.

Specifications

Product Overview
Quantity: 20 μg
Molecular Weight: 44 kDa to >150 kDa
Purity: >95% by SDS-PAGE
Substrate Properties: His6-tagged, N-terminus, working concentration from 0.5 µg to 2 µg
Storage Buffer: 50 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 2mM TCEP.
Storage Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.

Figures & Data

Ubiquitinated-p53, human recombinant

Figure 1. Ubiquitinated-p53 Western Blot. From left to right, Control His6-p53 (50 ng), ubiquitinated-p53 (50 ng and 100 ng), and ubiquitinated-p53 digested with USP7 (50ng).

Citations & References

1) Guo A., Salomoni P., Luo J., Shih A., Zhong S., Gu W., Pandolfi P.P. “The function of PML in p53-dependent apoptosis.” Nat. Cell Biol. 2:730-736(2000) 

2) Louria-Hayon I., Grossman T., Sionov R.V., Alsheich O., Pandolfi P.P., Haupt Y. “The promyelocytic leukemia protein protects p53 from Mdm2-mediated inhibition and degradation.” J. Biol. Chem. 278:33134-33141(2003)