His6-SARS-CoV (PLpro), SARS Coronavirus recombinant

Catalog number: SBB-DE0024, 50 μg

The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV His6-PLpro) is involved in the processing of the viral polyprotein. Proteolytic processing of the coronavirus replicase poly-protein is essential for generating a functional virus replication complex. PLpro is able to hydrolyze both ISG15-Rhodamine110 or diubiquitin/tetra-ubiquitin substrates, but is very inefficient when processing mono-Ub conjugates or synthetic peptide substrates. This PLpro is N-terminally His6 tagged and expressed in E.coli. [1][2][3][4]

In stock
SKU
0024
$149.00

Description

The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) is involved in the processing of the viral polyprotein. Proteolytic processing of the coronavirus replicase poly-protein is essential for generating a functional virus replication complex. PLpro possesses both deubiquitinating or deISGylating activity and can process Lys-48 and Lys-63 linked polyubiquitin chains (free chains or from cellular substrates). It works in concert together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. It strongly antagonizes the innate immune induction of type I interferon by blocking the phosphorylation, dimerization and therefore the nuclear translocation of host IRF3. In addition, it prevents also host NF-kappa-B signaling.

For Research Use Only, Not For Use In Humans.

Specifications

Product Overview
Quantity: 50 μg
Molecular Weight: 37.3 kDa
Purity: > 95% by SDS-PAGE
Substrate Properties: 6 Histidine tagged, N-terminus. Working concentrations of this enzyme range from 1 to 10 nM.
Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Storage Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.

Figures & Data

His6-PLpro, human recombinant

Figure 1. His6-PLpro, SDS-PAGE From left to right, increasing amounts of His6-PLpro loaded onto a 4-12% SDS-PAGE gel, stained with coomassie blue.

Citations & References

1) Barretto, N. et al. "The Papain-Like Protease Of Severe Acute Respiratory Syndrome Coronavirus Has Deubiquitinating Activity". Journal Of Virology, vol 79, no. 24, 2005, pp. 15189-15198. American Society For Microbiology, doi:10.1128/jvi.79.24.15189-15198.2005.

2) Ratia, K. et al. "Severe Acute Respiratory Syndrome Coronavirus Papain-Like Protease: Structure Of A Viral Deubiquitinating Enzyme". Proceedings Of The National Academy Of Sciences, vol 103, no. 15, 2006, pp. 5717-5722. Proceedings Of The National Academy Of Sciences, doi:10.1073/pnas.0510851103.

3) Rota, P. A. "Characterization Of A Novel Coronavirus Associated With Severe Acute Respiratory Syndrome". Science, vol 300, no. 5624, 2003, pp. 1394-1399. American Association For The Advancement Of Science (AAAS), doi:10.1126/science.1085952.

4) Ruan, Yijun et al. "Comparative Full-Length Genome Sequence Analysis Of 14 SARS Coronavirus Isolates And Common Mutations Associated With Putative Origins Of Infection". The Lancet, vol 361, no. 9371, 2003, pp. 1779-1785. Elsevier BV, doi:10.1016/s0140-6736(03)13414-9.