K6 Di-Ubiquitin

Catalog number: SBB-UP0060, 25 μg

This K6 linked di-ubiquitin was recombinantly expressed in E. coli, enzymatically conjugated, and purified via liquid chromatography.

In stock
SKU
0060
$95.00

Description

The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification. K6 linked ubiquitin linkages are found at increased levels in response to UV radiation, indirectly associated with DNA repair, and have been identified on mitochondrial outer membrane (MOM) proteins. This K6 linked di-ubiquitin was recombinantly expressed in E. coli, enzymatically conjugated, and purified via liquid chromatography.

For Research Use Only, Not For Use In Humans.

Specifications

Product Overview
Quantity: 25 μg
Molecular Weight: 17 kDa
Purity: >95% by SDS-PAGE
Storage Buffer: 50 mM HEPES pH 7.5
Storage Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw.

Figures & Data

K6-Linked Di-Ubiquitin, human recombinant

Figure 1. K6-Linked Di-Ubiquitin, SDS-PAGE From left to right, increasing amounts of di-ubiquitin loaded onto a 10-20% SDS-PAGE gel, stained with coomassie brillant blue. Purity is > 95%.

Citations & References

1) Dikic, I., Wakatsuki, S., & Walters, K. J. (2009). Ubiquitin-binding domains — from structures to functions. Nature Reviews Molecular Cell Biology, 10(10), 659–671. https://doi.org/10.1038/nrm2767

2) Akutsu, M., Dikic, I., & Bremm, A. (2016). Ubiquitin chain diversity at a glance. Journal of Cell Science, 129(5), 875–880. https://doi.org/10.1242/jcs.183954