K33 Di-Ubiquitin
Catalog number: SBB-UP0066, 25 μg
This K33 linked di-ubiquitin was recombinantly expressed in E. coli, enzymatically conjugated, and purified via liquid chromatography.
In stock
SKU
0066
$115.00
Description
The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification. This K33 linked di-ubiquitin was recombinantly expressed in E. coli, enzymatically conjugated, and purified via liquid chromatography.
For Research Use Only, Not For Use In Humans.
Specifications
| Quantity: | 25 μg |
|---|---|
| Molecular Weight: | 17 kDa |
| Purity: | >95% by SDS-PAGE |
| Storage Buffer: | 50 mM HEPES pH 7.5 |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw. |
Figures & Data
K33-Linked Di-Ubiquitin, human recombinant
Figure 1. K33-Linked Di-Ubiquitin, SDS-PAGE From left to right, increasing amounts of di-ubiquitin loaded onto a 10-20% SDS-PAGE gel, stained with coomassie brillant blue. Purity is > 95%.
Certificates of Analysis (COA)
Citations & References
1) Dikic, I., Wakatsuki, S., & Walters, K. J. (2009). Ubiquitin-binding domains — from structures to functions. Nature Reviews Molecular Cell Biology, 10(10), 659–671. https://doi.org/10.1038/nrm2767
2) Akutsu, M., Dikic, I., & Bremm, A. (2016). Ubiquitin chain diversity at a glance. Journal of Cell Science, 129(5), 875–880. https://doi.org/10.1242/jcs.183954
