20S Proteasome, rat RBC
Catalog Number: SBB-PP0086, 50 μg
Highly active protein complex that has been purified from rat red blood cells (RBC). The rat 20S proteasome is able to proteolytically degrade substrates in an ATP-independent manner. The 20S core can be activated chemically with SDS (0.035%) or by the addition of PA28. Initial experiments should be carried out at 20S proteasome concentrations between 2-5 nM.
The ubiquitin-proteasome pathway is the major proteolytic system in eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins or the rapid turnover of misfolded proteins. One of the most important proteases in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which is formed by the association of the barrel-shaped 20S proteasome (700-kDa) and two 19S (700-kDa) regulatory complexes. The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 20S catalytic core is able to degrade a variety of peptide substrates and poly-ubiquitinated proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control. The 20S proteasome can be activated chemically by the addition of the detergent SDS at a concentration not exceeding 0.035% or by the proteinaceous activator PA28.
|Molecular Weight:||>700 kDa|
|Purity:||>93% by SDS-PAGE|
|Storage Buffer:||50 mM HEPES pH 7.5, 100 mM NaCl,1 mM TCEP.|
|Storage||Store at -80 ̊C. Avoid multiple freeze thaw cycles.|
Certificates of Analysis (COA)
Citations & References
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- 2) Wilk S, Orlowski M (Mar 1983). “Evidence that pituitary cation-sensitive neutral endopeptidase is a multicatalytic protease complex”. Journal of Neurochemistry. 40 (3):842–9. doi:10.1111/j.1471-4159.1983.tb08056.x. PMID 6338156.
- 3) Haas AL, Warms JV, Hershko A, Rose IA (Mar 1982). “Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation”. The Journal of Biological Chemistry. 257(5): 2543–8. PMID 62779
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- 5) G.N. DeMartino & C.A. Slaughter (1999)”The proteasome, a novel protease regulated by multiplemechanisms”. J. Biol. Chem. 274, 22123 (1999) PMID: 10428